Gene therapy and viral vector-based vaccine manufacturers rely on basal media as the standard to grow their adeno-associated (AAV) viral vectors. The first-of-its-kind BalanCD HEK293 Viral Feed from FUJIFILM Irvine Scientific delivers a 3-fold to 10-fold increase in viral vector production over basal media alone to provide consistent performance, scalability, and speed time-to-market.

Figure 1. AAV2 viral genome titer and capsid titer were measured using cell extracts subjected to three cycles of freeze-thaw, followed by clarification by centrifugation and treatment with DNAse I and lysis buffer prior to qPCR (using AAVpro titration kit, Takara Bio) and ELISA (AAV2 titration ELISA, Progen). Blue and orange bars indicate the viral genome (VG) and capsid titer fold over the basal culture without a feed. The data indicates that the feed media enhancing the AAV2 production approximately 10-fold in VG and 6-fold for the capsid titer. As an overall result, viral packaging was determined to improve about 67%, from 13% in Basal to 21.81% in Basal+feed (data not shown). 

Figure 2. Verification of the BalanCD HEK293 Viral Feed performance on improving the production of AAV5 in 10L bioreactor scale. HEK293 suspension cells (cell line 2) were seeded at density of 0.8X10^6 cell/mL in 8.3 L culture volume in 10L bioreactor (XDR10 bioreactor). BalanCD HEK293 Viral Feed was added at 12% v/v at 24 hr post transfection. Titer was measured using ddPCR.