马来酰亚胺 PEG, mPEG-MAL


基本信息

产品名称 马来酰亚胺 PEG, mPEG-MAL
英文名称 Maleimide PEG, mPEG-MAL
规格或纯度 MW 5000 Da
运输条件 超低温冰袋运输
生化机理

Generally, a 10- to 20-fold molar excess of Maleimide PEG over the amount of thiol-containing material results in sufficient conjμgation.

Materials Required:

  • Pegylation buffer, PBS buffer, pH 7 or other thiol-free buffer at pH 7.
  • Maleimide stock solution: 100 mg in 1 mL conjμgation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjμgation buffer. Estimate the concentration thiol groups on the targeted materials. Add maleimide PEG stock solution to the targeted conjμgation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG maleimide needed for optimal conjμgation; Allow mixture agitates at room temperature for 2~4 hours at room temperature or overnight at 4 0C.Conjμgate can be purified either by size exclusion chromatography or dialysis.

一般描述

Maleimide functionalized polyethylene glycol, Maleimide PEG is a sulfhydryl (thiol/SH) group reactive PEG derivative that can be used to selectively modify protein, peptide or any other surfaces with availablesulfhydryl groups. The reaction between maleimide and thiol proceeds readily at pH 6.5~7.5 to form stable thioether bonds.

Maleimide functionalized polyethylene glycol, Maleimide PEG is a sulfhydryl (thiol/SH) group reactive PEG derivative that can be used to selectively modify protein, peptide or any other surfaces with availablesulfhydryl groups. The reaction between maleimide and thiol proceeds readily at pH 6.5~7.5 to form stable thioether bonds.

相关属性

敏感性 对空气敏感;对光敏感;对湿度敏感
溶解性 Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
储存温度 避光,-20°C储存,充氩
品牌 金畔生物