琥珀酰亚胺戊酸酯 PEG, mPEG-SVA

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基本信息

产品名称 琥珀酰亚胺戊酸酯 PEG, mPEG-SVA
英文名称 Succinimidyl valeric acid PEG, mPEG-SVA
规格或纯度 MW 5000 Da
运输条件 超低温冰袋运输
生化机理

Generally, a 10- to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjμgation.

Materials Required:

  • Pegylation buffer: PBS buffer, pH 7.4 or other amine-free buffer at pH 7-8.5.
  • PEG NHS stock solution: 50 mg in 1 mL conjμgation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjμgation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjμgation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C.Conjμgate can be purified either by size exclusion chromatography or dialysis.

*Note: Structure showed here mainly represents the functional groups derived from PEG. Exact linker may not show. Contact us if you have any concern about the linker.

一般描述

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol valeric acid (PEG-SVA) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl valeric acid PEG (mPEG-SVA) offers superior reactivity yet higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol valeric acid (PEG-SVA) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl valeric acid PEG (mPEG-SVA) offers superior reactivity yet higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

相关属性

敏感性 对湿度、光线敏感
溶解性 Off-white/white solid or viscous liquid depends on molecule weight;10 mg/mL, clear in water, DMSO, Chloroform;
储存温度 避光,-20°C储存,充氩
品牌 金畔生物