琥珀酰亚胺 PEG N-羟基琥珀酰亚胺, mPEG-NHS(SC)

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基本信息

产品名称 琥珀酰亚胺 PEG N-羟基琥珀酰亚胺, mPEG-NHS(SC)
英文名称 Succinimidyl PEG NHS, mPEG-NHS
规格或纯度 MW 10000 Da
运输条件 超低温冰袋运输
生化机理

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjμgation.

Materials Required:

  • Conjμgation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
  • PEG NHS stock solution: 100 mg in 1 mL conjμgation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjμgation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjμgation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjμgation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C. Conjμgates can be purified either by size exclusion chromatography or dialysis.

一般描述

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

相关属性

溶解性 Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
储存温度 -20°C储存
品牌 金畔生物