Why use the Silica Hydrogel for crystallization? Depending upon the macromolecule and selected conditions, gels can reduce nucleation and sedimentation, provide added stability, and allow crystals to grow larger. The porous network minimizes natural convection. Crystals are suspended in the gel network so that they do not form sediments and can grow free from strain exerted by the container or other crystals. The gel fissures around a growing crystal forming a cusp-like cavity. Heterogeneous and secondary nucleation are reduced in the presence of a silica hydrogel.³ The Silica Hydrogel can be used for liquid-gel, liquid-gel-liquid, vapor diffusion, as well as dialysis crystallization methodologies. The gel is compatible with a wide range of salts, polymers, organic solvents, and buffers used for macromolecular crystallization in a pH range from 3 to 10.

Each Silica Hydrogel kit contains twelve 500 microliter tubes of sodium silicate solution and twelve 500 microliter tubes of acetic acid solution. All solutions are formulated using ultra-pure water and are sterile filtered. Crystallization accessories are sold separately.

应用
用于凝胶结晶的快速简便的试剂盒形式
特征
具有中性 pH 值的专有二氧化硅水凝胶配方
凝胶基质可以减少成核和沉淀
描述
凝胶是用于生长大分子晶体的非常有效的介质。1-5 硅胶的优点尤其在于它们稳定、可在很宽的温度范围 (0-60°C) 内使用，并且与多种用于晶体生长的沉淀剂和添加剂。 Silica Hydrogel Kit 为您提供在室温下制备凝胶所需的材料，这些凝胶可快速聚合并形成 pH 值为 7.0 的多孔基质。中性 pH 值允许使用凝胶对沉淀剂的 pH 值影响最小。

为什么要使用二氧化硅水凝胶进行结晶？根据大分子和所选条件，凝胶可以减少成核和沉降，提供额外的稳定性，并使晶体长得更大。多孔网络最大限度地减少了自然对流。晶体悬浮在凝胶网络中，因此它们不会形成沉淀物，并且可以在不受容器或其他晶体施加的压力的情况下生长。凝胶在生长的晶体周围裂开，形成尖状腔。在二氧化硅水凝胶的存在下，异质和二次成核减少。3 二氧化硅水凝胶可用于液体凝胶、液体凝胶液体、蒸汽扩散以及透析结晶方法。该凝胶与各种盐、聚合物、有机溶剂和用于在 pH 值范围为 3 到 10 的大分子结晶中使用的缓冲液相容。

每个二氧化硅水凝胶套件包含十二个 500 微升的硅酸钠溶液管和十二个 500 微升的乙酸溶液管。所有溶液均使用超纯水配制并经过无菌过滤。结晶配件单独出售。

CAT NO

Silica Hydrogel kit