Samples include DNA-Drug complexes, C-Tetrad and G-Quartet Motifs, RNA oligomers, and others. By using 1 to 4 mM oligonucleotide stock concentration, the screen requires less than 100 µl of sample. The screen is typically performed at 4°C although room temperature incubations can also be performed. To evaluate the effect of equilibration kinetics as well as initial and final sample concentrations, the screens are typically performed using the hanging or sitting drop vapor diffusion method with two drops on a slide (1 µl + 2 µl and 2 µl + 2 µl), side by side. The 24 well format of the mini screen provides for a fast setup and uses small amounts of sample. This makes it possible to cost-effectively screen many sequences as well as variations of a particular sequence. The composition of the Nucleic Acid Mini Screen allows one to apply the formulation to other nucleic acids such as deoxy- and ribozymes, pseudoknots, and tRNAs.

Each Nucleic Acid Mini Screen kit (HR2-118) contains 24 unique reagents, 1.0 ml each. All solutions are formulated using ultra-pure Type 1 water and are sterile filtered. Dehydrants is available separately as 200 ml of 35% v/v (+/-)-2-Methyl-2,4-pentanediol in deionized water(catalog number HR2-863). Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL) and sterile filtered into sterile containers.

Note: Crystallization kit (HR2-118) and Dehydrant/reservoir solution (HR2-863) are sold separately. Both items are needed to perform the screen.

应用
核酸片段的结晶筛选
特征
筛选 pH、多胺和离子的矩阵
可用于核酶、假结、RNA 和 DNA
使用 MPD 作为主要沉淀剂
描述
Nucleic Acid Mini Screen 是一种高效筛选，用于帮助确定核酸片段的初步结晶条件。1 该配方基于出版物“A High Efficient 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments”，其中使用该配方确定了 35 种核酸的初步结晶条件。 1

样品包括 DNA-药物复合物、C-Tetrad 和 G-Quartet Motifs、RNA 寡聚体等。通过使用 1 至 4 mM 寡核苷酸储备浓度，筛选所需的样品少于 100 µl。筛选通常在 4°C 下进行，但也可以在室温下进行孵育。为了评估平衡动力学以及初始和最终样品浓度的影响，通常使用悬滴或坐滴蒸汽扩散法在载玻片上滴两滴（1 µl + 2 µl 和 2 µl + 2 µl）进行筛选，并排。迷你屏幕的 24 孔格式提供快速设置并使用少量样品。这使得经济高效地筛选许多序列以及特定序列的变体成为可能。 Nucleic Acid Mini Screen 的组成允许将配方应用于其他核酸，例如脱氧酶和核酶、假结和 tRNA。

每个 Nucleic Acid Mini Screen 试剂盒 (HR2-118) 包含 24 种独特的试剂，每种试剂 1.0 ml。所有溶液均使用超纯 1 类水配制并经过无菌过滤。脱水剂可单独以 200 毫升 35% v/v (+/-)-2-甲基-2,4-戊二醇在去离子水中的形式提供（目录号 HR2-863）。在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，< 5 ppb 总有机碳，无细菌（<1 细菌（CFU/ml）），无热原（<0.03 内毒素（EU/ml）） , RNase-free (< 0.01 ng/mL) 和 DNase-free (< 4 pg/µL) 和无菌过滤到无菌容器中。

注意：结晶试剂盒 (HR2-118) 和脱水剂/贮液器溶液 (HR2-863) 单独出售。执行屏幕需要这两个项目。

 

Nucleic Acid Mini Screen

(+/-)-2-Methyl-2,4-pentanediol

 HR2-118 Nucleic Acid Mini Screen Documents HR2-118 Nucleic Acid Mini Screen SDS HR2-863 (+/-)-2-Methyl-2,4-pentanediol SDS Nucleic Acid Mini Screen Formulation & Scoring

1. Berger, et al., A Highly Effective 24 Condition Matrix for the Crystallization of Nucleic Acid Fragments. Acta Cryst. Section D. (1996) Vol. D52 Part 3, 465-468.

2. Adams, A., Nucleic Acids Research (2002) Vol. 30, 719-725.

3. Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNAGly acceptor-stem microhelix. C. Forster, M. Perbandt, A. B. E. Brauer, S. Brode, J. P. Furste, C. Betzel and V. A. Erdmann. Acta Cryst. (2007). F63, 46-48.