Cygnus CHO HCP ELISA Kit, 3G (F550)中文说明书
This product has been depleted and replaced with CHO HCP ELISA Kit, 3G (F550-1)
该产品已用完并替换为 CHO HCP ELISA Kit, 3G (F550-1)
由于 F550 的捕获和检测抗体库存耗尽，我们已重新供应。 如果您有任何问题或需要进一步帮助。
Product Information
Product Type: ELISA Kit
Storage: All kit reagents 2°C to 8°C
Target Expression System: CHO
Species Group: Mammalian
Species: Chinese hamster
产品信息
产品类型：ELISA 试剂盒
储存：所有试剂盒试剂 2°C 至 8°C
目标表达系统：CHO
物种组：哺乳动物
种类：中国仓鼠
Description
Important Change Notification Memo: CHO HCP ELISA Kit, 3G Resupply
New CHO HCP ELISA Kit, 3G, Item Number F550-1 now available
This CHO HCP 3rd Generation ELISA Kit uses a more broadly reactive antibody than our earlier kits and incorporates a number of other improvements for the detection of CHO HCPs. The “CHO 3G” ELISA is more sensitive than previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. It has been extensively qualified with a large number of drug substances and in-process samples. This data indicates the kit is truly a generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay. The antibody has been evaluated for reactivity to individual HCPs using the conventional 2D Western Blot as well as a method we have shown to be superior to 2D WB, which we call AAE, Antibody Affinity Extraction. AAE demonstrated the antibody is reactive to more than 1000 individual HCPs from conditioned media and cell lysates in both a CHO-S strain as well as a K1 strain. These 1000+ HCPs represent more than 98% of the total mass of protein as indicated by protein detection methods orthogonal to ELISA. As such, this antibody may overcome concerns about missing any significant individual HCPs. This ELISA is also more robust than the earlier kits showing less sample matrix interference and improved dilution linearity. If you have found the F015 and CM015 kits lacking in any way meeting your HCP detection needs, or if you are considering the need for a process-specific assay, we recommend you evaluate this 3rd Generation CHO HCP ELISA kit.
描述
重要变更通知备忘录：CHO HCP ELISA 试剂盒、3G 补给
新的 CHO HCP ELISA 试剂盒，3G，货号 F550-1 现已上市
此 CHO HCP 第三代 ELISA 试剂盒使用比我们早期试剂盒更广泛的反应性抗体，并结合了许多其他改进来检测 CHO HCP。 “CHO 3G” ELISA 比以前的试剂盒更敏感，能够检测 CHO 中表达的各种抗体和其他治疗性蛋白的十亿分之 100 范围内的 HCP。它已通过大量原料药和在制品样品进行了广泛的鉴定。该数据表明该试剂盒确实是一种通用方法，不仅可以作为工艺开发工具进行验证，而且在大多数情况下可以作为批量放行测试，从而无需开发定制或工艺特定的检测方法。已使用传统的 2D Western Blot 以及我们已证明优于 2D WB 的方法（我们称之为 AAE，抗体亲和提取）评估了该抗体对单个 HCP 的反应性。 AAE 证明该抗体对来自 CHO-S 菌株和 K1 菌株的条件培养基和细胞裂解物的 1000 多个单独的 HCP 具有反应性。与 ELISA 正交的蛋白质检测方法表明，这 1000 多个 HCP 占蛋白质总质量的 98% 以上。因此，这种抗体可以克服对遗漏任何重要个体 HCP 的担忧。这种 ELISA 也比早期的试剂盒更稳健，显示出更少的样品基质干扰和改进的稀释线性。如果您发现 F015 和 CM015 试剂盒在任何方面都无法满足您的 HCP 检测需求，或者如果您正在考虑需要特定过程的检测，我们建议您评估这款第三代 CHO HCP ELISA 试剂盒。
Kit Components 试剂盒组成
Anti-CHO:HRP Conjugate, 3G
CHO HCP Standards Set, A-F, 3G
Stop Solution
TBS Wash Concentrate, 20X
TMB Substrate for ELISA
Anti-CHO Coated Microtiter Strips
Product FAQs 产品问与答
Why Perform Dual Wavelength Analysis?
The protocols in our ELISA kits recommend the use of dual wavelength analysis for microtiter plates readers appropriately equipped. For alkaline phosphatase – PNPP based assays the two wavelengths are 405 nm and 492 nm. For horse radish peroxidase – TMB based assays the wavelengths are 450 nm and 630 nm. In both assay types the first wavelength listed, also termed the “test wavelength”, is critical and thus your microtiter plate reader should be equipped with the appropriate 405 nm and 450 nm filters. It is not absolutely necessary to use dual wavelength analysis with any of our assays and thus the second wavelength (492 nm and 630 nm – also called the “reference wavelengths”) are optional. In theory, the use of dual wavelength analysis should provide better precision. Microtiter plate readers with the appropriate software will automatically subtract the reference wavelength absorbance from the test wavelength absorbance. In theory dual wavelength analysis can help to overcome any non-wavelength specific imperfections in the plate. In practice, because the optical quality of our microtiter plates is excellent, there is usually no significant statistical improvement in the data by subtracting out the reference absorbance from the test absorbance. Most plate readers are equipped to perform dual wavelength analysis. Since the reference wavelength is not critical, microtiter plate readers with reference filters close to those we recommend can be substituted. For example, you may use a 490 nm or a 500 nm filter in place of the 492 nm or a 600 nm or 650 nm filter in place of the 630 nm filter.
为什么要进行双波长分析？
我们的 ELISA 试剂盒中的方案建议对适当配备的微量滴定板读数器使用双波长分析。对于碱性磷酸酶 – 基于 PNPP 的分析，两个波长是 405 nm 和 492 nm。对于辣根过氧化物酶 – 基于 TMB 的检测，波长为 450 nm 和 630 nm。在这两种检测类型中，列出的第一个波长（也称为“测试波长”）至关重要，因此您的微量滴定板读数器应配备适当的 405 nm 和 450 nm 滤光片。在我们的任何检测中使用双波长分析并不是绝对必要的，因此第二个波长（492 nm 和 630 nm – 也称为“参考波长”）是可选的。理论上，使用双波长分析应该提供更好的精度。带有适当软件的微量滴定板读数器会自动从测试波长吸光度中减去参考波长吸光度。理论上，双波长分析可以帮助克服印版中任何非波长特定的缺陷。在实践中，由于我们的微量滴定板的光学质量非常好，从测试吸光度中减去参考吸光度通常不会对数据进行显着的统计改进。大多数读板机都配备执行双波长分析。由于参考波长并不重要，因此可以替换带有接近我们推荐的参考滤光片的微量滴定板读数器。例如，您可以使用 490 nm 或 500 nm 滤光片代替 492 nm 或 600 nm 或 650 nm 滤光片代替 630 nm 滤光片。
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