Vectorlabs 马抗山羊IgG Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

Vectorlabs 马抗山羊IgG Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

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ABC免疫组化试剂盒、ImmPRESS聚合物免疫组化试剂盒、防荧光淬灭封片剂、二抗、酶底物、亲和素/链霉亲和素-酶结合物、封闭试剂。

Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg

品牌:Vectorlabs
产品货号:PI-9500-1
规格:1 mg
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
  • 产品名称:
    Horse Anti-Goat IgG Antibody (H+L), Peroxidase, 1 mg
  • 别名:
    马抗山羊IgG(H+L)抗体,HRP标记
  • 产品描述:
  • Horse Anti-Goat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.As well as recognising goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies.
  • Horse Anti-Goat IgG Antibody 通过亲和层析纯化并吸附以去除任何可能干扰特定标记的交叉反应性。 然后该抗体与最高比活性的辣根过氧化物酶交联。 专有的偶联程序可最大限度地保留酶活性和抗体特异性。 该抗体经过严格的质量控制检测,可用于组织染色、ELISA 和印迹。除识别山羊 IgG 外,该抗体还适用于检测绵羊和牛 IgG 一抗。
  • 反应种属:
    Goat
  • 宿主来源:
    Horse
  • 实验应用:
    Immunohistochemistry / Immunocytochemistry,In situ hybridization,Blotting Applications,Elispot,ELISAs
  • 推荐稀释度:
  • Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in thse applications, blocking with Animal-Free blocker‚Ñ¢ solution (Cat. No. SP-5030) is recommended. Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti goat IgG is 0.5-5.0µg/ml.
  • 重要提示:在 ELISA 或印迹分析中使用牛产品(如酪蛋白、血清、白蛋白或脱脂奶粉)作为封闭剂可能会产生高背景,因为抗山羊 IgG 与牛免疫球蛋白可能会产生交叉反应。 如果要在这些应用中使用该抗体,建议使用无动物阻滞剂,Ñ¢ 溶液(货号 SP-5030)进行封闭。 推荐用于微量滴定板测定的稀释剂:10 mM 磷酸盐、0.15 M NaCl、pH 7.5、0.1% Tween 20。HRP-抗山羊 IgG 的工作浓度为 0.5-5.0µg/ml。
  • 偶联:
    Peroxidase
  • 产品浓度:
    1 mg active conjugate/ml
  • 产品形式:
    Concentrate
  • 存储溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
  • 样本类型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative