Hyaluronic acid (HA) can be obtained from various sources, for example rooster combs orStreptococcus equi. We can supply derivatives of either of these qualities labelled with tetramethylrhodamine (T-HA). All batches are checked for molecular weight, degree of substitution, loss on drying and free TRITC. TdB Labs can produce T-HA with a molecular weight of approximately 1.5 MDa. T-HA is supplied as a pink powder.

透明质酸 (HA) 可以从各种来源获得，例如鸡冠或马链球菌。 我们可以提供标有四甲基罗丹明 (T-HA) 的这些品质的衍生物。 检查所有批次的分子量、取代度、干燥失重和游离 TRITC。 TdB Labs 可以生产分子量约为 1.5 MDa 的 T-HA。 T-HA 以粉红色粉末形式提供。

Structure 结构
Hyaluronic acid is a polysaccharide composed of alternating β(1-3) glucuronide and β(1-4) glucosaminide units derived from Streptococcus equi. The hyaluronic acid is labelled with amino-tetramethylrhodamine. The degree of substitution lies between 0.001 and 0.008. The labelling procedures do not lead to depolymerisation of the HA.

透明质酸是一种多糖，由来自马链球菌的交替的 β(1-3) 葡糖苷酸和 β(1-4) 葡糖胺单位组成。 透明质酸用氨基四甲基罗丹明标记。 取代度介于 0.001 和 0.008 之间。 标记程序不会导致 HA 解聚。

Spectral data
T-HA has an excitation maximum at 552 nm and an emission maximum at 576 nm.

光谱数据
T-HA 在 552 nm 处具有最大激发，在 576 nm 处具有最大发射。

Storage and stability 
T-HA is stable for more than 6 years when stored dry in well-sealed containers.

储存和稳定性
在密封良好的容器中干燥储存时，T-HA 可稳定保存 6 年以上。

Solubility
T-HA dissolves readily in water.

溶解度
T-HA 易溶于水。

Application 
T-HA have been used to e.g. investigate invasive growth into brain tissue. Read more about applications here.

应用
T-HA 已被用于例如 研究侵入性生长进入脑组织。 在此处阅读有关应用程序的更多信息。

References

1. Pusch, A. et al. CD44 and hyaluronan promote invasive growth of B35 neuroblastoma cells into the brain. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research 1803, 261–274 (2010)
2. Kaminski, T., Siebrasse, J.-P., Gieselmann, V., Kubitscheck, U., Kappler, J. Imaging and tracking of single hyaluronan molecules diffusing in solution. Glycoconjugate Journal 25(6), 555-560 (2008).