FITC-lysine-dextran is a derivative carrying both free primary amines and free carboxylates as well as the fluorescent dye Fluroescein (FITC). Lysine is a naturally occurring amino acid and popular moiety for conjugation and fixation in biochemistry and life sciences.
FITC-lysine-dextran is also known as Fluoro-emerald® or fluoroemerald ®. After purification, all batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. FITC-lysine-dextrans are supplied by TdB Labs as a yellow to orange powder TdB Labs with a molecular weight from 4 kDa to 500 kDa.
Fluorescein (FITC) is a bright dye that is used world-wide and absorbs at 493 nm and emits light at 520 nm.

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Structure
FITC-lysine-dextrans are synthesised from well-characterised dextran fractions derived from Leuconostoc mesenteroides. The dextran fractions are first labelled with lysine and thereafter functionalised with FITC under mild conditions to form FITC-lysine-dextran. The degree of substitution of lysine is 0.005-0.03 (mol lysine/mol glucose) and the degree of substitution of FITC is 0.001-0.01 (mol FITC/mol glucose).

Spectral data
The excitation maximum for FITC-lysine-dextran is 493 nm and the emission maximum is 520 nm.

Storage and stability
FITC-lysine-dextran is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.

Solubility
FITC-lysine-dextran dissolves readily in water.

Application
FlTC-lysine-dextran is a versatile tool for bioconjugation and fixation in living systems. Read more about applications here.

References

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How to order

Visit our webshop to see the molecular weights and pack sizes available. Please send an e-mail to order@tdblabs.se if you would like to receive a quote, place a bulk order or if you wish to place your order manually.