MegazymeL-天门冬素[天冬酰胺酸]/L-谷氨酸/氨检测试剂盒
上海金畔生物科技有限公司
品牌:Megazyme
英文名:L-Asparagine/L-Glutamine/ Ammonia
货号:K-ASNAM
规格:100 Assays (50 of each)
分析物意义: 炸、烘、烤土豆或其他食品中的丙烯酰胺的前提
Megazyme检测试剂盒优点:产品新颖,不到20分钟可以检测3种组分。可用于手工或酶标仪自动检测。
The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of L-Asparagine, L-Glutamine
and Ammonia in potatoes, foodstuffs and cell culture media
Principle:
(glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+
(microbial glutamate dehydrogenase)
(2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O
(asparaginase)
(3) L-Asparagine + H2O → L-aspartate + NH4+
Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 20 min
Detection limit: 0.50 mg/L (L-asparagine)
0.54 mg/L (L-glutamine)
0.06 mg/L (ammonia)
Application examples:
Potatoes, potato products, vegetables, cereals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition: Novel method
Advantages
- Very rapid reaction due to use of uninhibited glutamate dehydrogenase
- All enzymes supplied as stabilised suspensions
- Only kit available
- Very cost effective
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
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件
Megazyme产品所有分类:
生物及食品酶法试剂盒(Assay Kits)
膳食纤维/淀粉(Dietary Fiber/Starch) 单/双糖(Mono/Disaccharides) 多糖(Polysaccharides) 醇类(Alcohols) 有机酸(Organic Acids) 亚硫酸盐/氮(Sulfite/Nitrogen) 活性酶(Enzyme Activity) 试剂混合物(Reagent Mixtures) 其它(Other)
酶(Enzymes)
活性酶(Carbohydrate Active enZYmes )
淀粉酶(Amylases )
阿拉伯树胶酸(Arabinanases)
阿拉伯呋喃(Arabinofuranosidases)
纤维二糖水解(Cellobiohydrolases)
纤维素酶(Cellulases)
酯酶(Esterases)
果糖酶&果糖苷酶(Fructanases & Fructosidases)
岩藻糖苷酶(Fucosidases)
半乳聚糖酶(Galactanases)
半乳糖苷酶(Galactosidases)
葡聚糖酶(Glucanases)
葡萄糖苷酶(Glucosidases)
葡糖醛酸糖苷(Glucuronidases)
已糖胺酶类(Hexosaminidiases)
裂解酶(Lyases)
甘露聚糖酶(Mannanases)
甘露糖苷酶(Mannosidases)
支链淀粉酶(Pullulanases)
唾液酸酶(Sialidases)
木聚糖酶(Xylanases)
糖苷酶(Xylosidases)
木葡聚糖酶(Xyloglucanases)
多聚半乳糖醛酸(内切)酶(Polygalacturonases)
其它酶(Other Activities)
分析酶(Analytical Enzymes)
脱氢酶(Dehydrogenases)
异构酶(Isomerases)
激酶(Kinases)
磷酸酶(Phosphatases)
蛋白酶(Proteases)
其他活性酶(Other Activities)
糖类活性酶(Glycobiology Enzymes)
酶底物( Chromogenic Substra