1、概述

NAP DNA Purification Columns Disposable NAP DNA purification columns, prepacked with Sephadex G-25 DNA Grade for gravity purification of nucleic acids (≥ 10mers).

For rapid and efficient purification of DNA and oligonucleotides (≥ 10-mers) in less than 15 minutes by gravity flow Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG/ICP Biocide Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL (NAP-25) Useful for small-scale purification, oligo purification, desalting, and buffer exchange NAP DNA purification columns with Sephadex G-25 DNA Grade can be used for any DNA greater than 10 bases in length.

These disposable DNA purification columns are ideal for the purification of oligonucleotides or very small DNA fragments following synthesis or a DNA labeling reaction, and for downstream applications such as PCR amplification and sequencing. Each package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration, washing, and sample elution. Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules.

Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length. Other Sephadex DNA purification columns and resins, and other DNA purification supports are also available depending on your exact application.

一、概述

NAP DNA 纯化柱 一次性 NAP DNA 纯化柱，预装 Sephadex G-25 DNA Grade，用于核酸（≥ 10mers）重力纯化。

通过重力流在不到 15 分钟的时间内快速有效地纯化 DNA 和寡核苷酸（≥ 10 聚体） 预装 Sephadex G-25 DNA 级在含有 0.15% Kathon CG/ICP 杀菌剂的蒸馏水中 根据样品体积提供三种尺寸: 0.5 mL (NAP-5)、1 mL (NAP-10) 或 2.5 mL (NAP-25) 适用于小规模纯化、寡核苷酸纯化、脱盐和缓冲液交换 NAP DNA 纯化柱与 Sephadex G-25 DNA Grade可用于长度超过 10 个碱基的任何 DNA。

这些一次性 DNA 纯化柱非常适合在合成或 DNA 标记反应后纯化寡核苷酸或非常小的 DNA 片段，以及用于 PCR 扩增和测序等下游应用。每个包都包含一个完整的分步方案，描述了平衡、洗涤和样品洗脱所需的固定体积。 Sephadex G-25 是五种不同的 G 型之一，范围从用于小分子的 G-10 到用于较大分子的 G-75。

Sephadex G-25 的排阻极限约为 Mr 5000，当装入空的 MicroSpin 柱时，所得脱盐柱可用于 DNA 和寡核苷酸纯化任何长度超过 10 个碱基的 DNA 的离心方案。根据您的具体应用，还可提供其他 Sephadex DNA 纯化柱和树脂以及其他 DNA 纯化支持物。