1.概述

Sensor Chip CM5 Supports an exceptional range of immobilization chemistries and applications including ligand fishing, target validation & quality control.Biacore Sensor Chip CM5 supports a range of immobilization chemistries to capture nucleic acids, peptides, antibodies, proteins, carbohydrates, or chemical compounds for interaction analysis from research to discovery, development & quality control.

First choice for immobilization via -NH2, -SH, -CHO, -OH, or -COOH groups. Attach proteins, antibodies, nucleic acids, carbohydrates, and small molecules (<1000 Da). Compatible with a wide range of applications from basic research to quality control.

Suitable for ligand fishing and fragment-based drug discovery (FBDD). High immobilization capacity permits a broad range of capture densities.

Obtain real-time data on binding specificity, affinity, kinetics, concentration and more. Biacore Sensor Chip CM5 Matrix The CM5 sensor chip carries a matrix of carboxymethylated dextran covalently attached to a gold surface. Molecules can be covalently coupled to the sensor surface by exploiting available amine, thiol, aldehyde, or carboxyl functional groups on the ligand. The CM5 dextran matrix extends about 100 nm from the gold surface, and is flexible, allowing relatively free movement of attached ligands. An exceptionally versatile chip for SPR interaction analysis Sensor Chip CM5 is the most versatile and widely used dextran-based chip, suitable for most general-purpose surface plasmon resonance (SPR) applications.

Carboxymethyl surface chemistry supports the study of many different types of ligands and binding partners–from small organic molecules, such as drug candidates, through to large molecular assemblies and even whole viruses.

High binding capacity gives a high response, advantageous for capture assays and for interactions involving small molecules. High surface stability provides accuracy and precision, and allows repeated analysis on the same surface.

Application examples include: FBDD-based screening for novel drugs against Hepatitis C virus (HCV) Surface plasmon resonance-based determination of adenovirus concentration on Biacore T200 Sensor chip formats for Biacore systems Biacore sensor chips are available in two formats: “S series” sensor chips are for use with Biacore 8K+, Biacore 8K, Biacore S200, Biacore T200, and Biacore 4000 SPR systems. Sensor chips not designated with “S series” are designed for Biacore X100, Biacore 3000 and Biacore C SPR systems.

1.概述

传感器芯片 CM5 支持一系列特殊的固定化学和应用，包括配体捕获、目标验证和质量控制。 Biacore 传感器芯片 CM5 支持一系列固定化学以捕获核酸、肽、抗体、蛋白质、碳水化合物或用于相互作用的化合物从研究到发现、开发和质量控制的分析。

通过 -NH2、-SH、-CHO、-OH 或 -COOH 基团进行固定的首选。连接蛋白质、抗体、核酸、碳水化合物和小分子 (<1000 Da)。兼容从基础研究到质量控制的广泛应用。

适用于配体捕鱼和基于片段的药物发现 (FBDD)。高固定能力允许广泛的捕获密度。

获取有关结合特异性、亲和力、动力学、浓度等的实时数据。 Biacore 传感器芯片 CM5 矩阵 CM5 传感器芯片带有一个共价连接到金表面的羧甲基化葡聚糖矩阵。通过利用配体上可用的胺、硫醇、醛或羧基官能团，分子可以共价偶联到传感器表面。 CM5 葡聚糖基质从金表面延伸约 100 nm，是灵活的，允许连接的配体相对自由移动。用于 SPR 相互作用分析的极其通用的芯片 传感器芯片 CM5 是用途最广、使用最广泛的基于葡聚糖的芯片，适用于大多数通用表面等离子体共振 (SPR) 应用。

羧甲基表面化学支持对许多不同类型的配体和​​结合配偶体的研究——从有机小分子（如候选药物）到大分子组装体甚至整个病毒。

高结合能力提供高响应，有利于捕获分析和涉及小分子的相互作用。高表面稳定性提供准确度和精密度，并允许在同一表面上重复分析。

应用示例包括： 基于 FBDD 筛选针对丙型肝炎病毒 (HCV) 的新型药物 Biacore T200 上基于表面等离子体共振的腺病毒浓度测定 Biacore 系统的传感器芯片格式 Biacore 传感器芯片有两种格式：“S 系列”传感器芯片用于 Biacore 8K+、Biacore 8K、Biacore S200、Biacore T200 和 Biacore 4000 SPR 系统。未标明“S 系列”的传感器芯片专为 Biacore X100、Biacore 3000 和 Biacore C SPR 系统设计。