1.概述

HiTrap Fibro™ and HiScreen Fibro™ PrismA protein A chromatography Protein A fiber chromatography unit for rapid cycling chromatography in research and process development applications.HiTrap Fibro™ and HiScreen Fibro™ PrismA are protein A fiber chromatography units for rapid cycling chromatography purification of mAbs for research and process development applications.

Ultrafast purification enables cycle times under 5 minutes compared to hours for resins. HiTrap Fibro™ PrismA is primarily intended for reserach use and HiScreen Fibro™ PrismA is primarily intended for early process development. Purify mAbs and Fc-containing fragments using rapid cycling chromatogrpaphy.

Cycle times are less than 5 minutes compared with hours for chromatography resins. Increase throughput up to 20-fold over that for resin-based chromatography and perform a full life time study in one day. This cuts weeks from lead times in process development. Achieve high-throughput purification of up to 500 mAbs per week for cell line or candidate screening.

Run Fibro PrismA units on an ÄKTA™ chromatography system for real-time UV, pH and conductivity detection. The HiTrap Fibro™ and HiScreen Fibro™ PrismA adsorbents have a protein A cellulose fiber matrix with an open pore structure where mass transfer is governed by convective flow.

This structure allows high mAb binding capacities at very short residence times by using rapid cycling chromatography. This results in cycle times of minutes instead of the hours needed for resin-based chromatography. Dynamic binding capacity is ~ 30 mg IgG/mL matrix. The Fibro technology platform is designed to scale up to manufacturing scale.

The units will range from research scale with a capacity of approximately 12 mg per purification cycle (0.4 mL units) to GMP compatible units that can process up to 2000 L bioreactor harvest or 10 kg mAb in one working day by cycling multiple times. Applications Intensify protein A chromatography. Overcome chromatography challenges. Increase throughput with this automated two-step process.

Cut lead times dramatically using high-throughput process development. Customer Case study: High throughput mAb purification. 14 questions of Fibro chromatography. Getting started Using resin-based columns? Fibro PrismA units connect to ÄKTA™ systems in the same way. And you can use the same buffers. Free E-learning: Getting started with Fibro

HiTrap Fibro™ 和 HiScreen Fibro™ PrismA 蛋白 A 层析 蛋白 A 纤维层析装置，用于研究和工艺开发应用中的快速循环层析。HiTrap Fibro™ 和 HiScreen Fibro™ PrismA 是蛋白 A 纤维层析装置，用于研究用 mAb 的快速循环层析纯化和工艺开发应用。

与树脂的数小时相比，超快速纯化可将循环时间缩短到 5 分钟以内。 HiTrap Fibro™ PrismA 主要用于研究用途，而 HiScreen Fibro™ PrismA 主要用于早期工艺开发。使用快速循环色谱法纯化 mAb 和含 Fc 的片段。

与色谱树脂的数小时相比，循环时间少于 5 分钟。将通量提高至基于树脂的色谱的 20 倍，并在一天内完成全生命周期研究。这将流程开发的交付周期缩短了数周。实现每周高达 500 mAb 的高通量纯化，用于细胞系或候选筛选。

在 ÄKTA™ 色谱系统上运行 Fibro PrismA 装置，以进行实时 UV、pH 和电导检测。 HiTrap Fibro™ 和 HiScreen Fibro™ PrismA 吸附剂具有蛋白质 A 纤维素纤维基质，该基质具有开孔结构，其中传质受对流控制。

通过使用快速循环色谱，这种结构可以在非常短的停留时间内实现高 mAb 结合能力。这导致循环时间为几分钟，而不是基于树脂的色谱法所需的数小时。动态结合容量为 ~ 30 mg IgG/mL 基质。 Fibro 技术平台旨在扩大到制造规模。

这些装置的范围从每个纯化循环容量约为 12 毫克（0.4 毫升单位）的研究规模到 GMP 兼容装置，通过多次循环，可以在一个工作日内处理多达 2000 L 生物反应器收获或 10 kg mAb。应用 加强蛋白质 A 层析。克服色谱挑战。通过这种自动化的两步流程提高吞吐量。

使用高通量工艺开发显着缩短交货时间。客户案例研究：高通量 mAb 纯化。纤维色谱的14个问题。使用入门 使用树脂柱？ Fibro PrismA 装置以相同的方式连接到 ÄKTA™ 系统。您可以使用相同的缓冲区。免费在线学习：Fibro 入门

2.规格