1.概述

Ni Sepharose excel histidine-tagged protein purification resin Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents. Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.

Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping. Retained binding capacity of up to 10 mg/mL resin. Simplified sample handling – fewer steps needed compared with conventional workflow.

Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification. EDTA-resistant resin for IMAC chromatography Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA.

Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.

His tag purification of large sample volumes The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.

Successful use in published research Scientists use Ni Sepharose excel to purify his-tagged recombinant proteins from eukaryotic and prokaryotic samples, as cited in these publications: Human transferrin receptor 1 secreted from BHK cells Proprotein convertase subtilisin/kexin type 9 from HEK293 supernatants Membrane protein insertase EcYidC from E. coli for crystallization Helicobacter pylori adhesin BabA from periplasmic extracts Convenient prepacked columns Ni Sepharose excel resin is also available in the following formats: HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.

His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.

Ni Sepharose excel 组氨酸标签蛋白质纯化树脂 Ni Sepharose excel 是一种组氨酸标签蛋白质纯化树脂，可在样品含有剥离剂时最大限度地减少镍泄漏并最大限度地提高蛋白质回收率。多功能——适用于真核样品，包括昆虫和 CHO 细胞，以及细菌样品，如大肠杆菌。

直接上样 – 真核细胞培养上清液，无需对通常会导致镍剥离的样品进行预处理。保留高达 10 mg/mL 树脂的结合容量。简化样品处理——与传统工作流程相比，所需步骤更少。

灵活的形式——预装和松散的树脂形式，用于优化筛选和制备型 His 标签纯化。用于 IMAC 层析的抗 EDTA 树脂 Ni Sepharose excel 是一种 IMAC 树脂，其中预装了与螯合配体非常牢固地结合的镍离子。即使在 10 mM EDTA 中孵育 24 小时后，镍离子仍与配体结合。

由于这种强结合，通常会导致金属离子剥离的样品可以直接加载到树脂上，而无需耗时的预处理。因此，这种his标签纯化树脂特别适用于纯化分泌到真核细胞培养上清液中的组氨酸标签（his-tagged）蛋白。

大体积样品的 His 标记纯化 Ni Sepharose excel 镍树脂的流动特性使其非常适合各种规模的纯化。这种树脂可以处理大量样品的加载，因此您可以使用手动或自动方法纯化低浓度的目标蛋白质。

在已发表的研究中成功应用 科学家们使用 Ni Sepharose excel 从真核和原核样品中纯化带有 His 标签的重组蛋白，如这些出版物中所引用的： 从 BHK 细胞分泌的人转铁蛋白受体 1 来自 HEK293 上清液的 Proprotein convertase subtilisin/kexin type 9 膜蛋白插入酶来自大肠杆菌的 EcYidC 用于结晶 来自周质提取物的幽门螺杆菌粘附素 BabA 方便的预装柱 Ni Sepharose excel 树脂也有以下形式： HisTrap excel 柱：HiTrap 形式，便于与注射器、蠕动泵或色谱系统一起使用.

His MagSepharose excel：用于小规模纯化和筛选的磁珠。当需要许多平行实验时，它们适用于研究的早期阶段。

2.规格