Wako Anti Iba1, Monoclonal Antibody (NCNP24)

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Wako Anti Iba1, Monoclonal Antibody (NCNP24)

Iba1 is a protein highly expressed in microglia and macrophage with a molecular weight of about 17 kDa. The protein is a commonly known as a microglial marker in the central nervous system.
Recently, microglia has been extensively studied because they are responsible for immune function in the central nervous system, and are related to various diseases such as neurodegenerative diseases, mental illuness, brain tumors and infection.

  • 1. LINK
  • 2. Features
  • 3. Applications: Immunohistostaining
  • 4. Antibody information
  • 5. Product List
  • 6. Related Products

Wako Anti Iba1, Monoclonal Antibody (NCNP24)

1. LINK

  • Leaflet  (1,066KB/2p)
  • Anti Iba1, Rabbit, Polyclonal antibodies ⇒ LINK
  • Labeled Iba1 Antibodies ⇒ LINK
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2. Features

  • Evaluated by immunohistochemistry
  • Useful for multicolor immunostaining
  • Monoclonal antibody with high specificity and low background
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3. Applications – Immunohistostaining –

Application-Immunohistostaining -

                        → Clear localization signals of microglia using Anti Iba1, Monoclonal Antibody (Wako Cat. No.012-26723)
as well as using Anti Iba1, Rabbit polyclonal antibody (Wako Cat. No.019-19741 019-19741).

♦ Assay Condition:

· Sample: cerebral cortex of 7-week Wistar rat, 7-week ICR mouse, or adult marmoset

· Section: 50μm-frozen section (mouse and rat), 40μm-frozen section (marmoset)

· Staining method: ABC method + DAB staining

· Antibody concentration = 1: 500

All immunohistochemistry data were provided by Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

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4. Antibody information

Anti Iba1, Monoclonal Antibody (NCNP24)
Antigen Synthetic peptide (C-terminal of Iba1)
Clone No. NCNP24
Subclass Mouse IgG1
Concentration 1.3 mg/mL (the first lot)
Buffer 50% Glycerol / TBS, 0.05% Sodium Azide
Species reactivity Mouse, Rat, Marmoset
Application Immunohistochemistry (frozen section) 1:500 – 2,000)
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5. Product List

Product Name Pkg. Size Wako Cat. No. Storage Condition
Anti Iba1, Monoclonal Antibody (NCNP24) 10 μL 012-26723 Keep at -20°C.
50 μL 016-26721
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6. Related Products

Product Name Pkg. Size Wako Cat. No. Storage Condition
Anti Iba1, Rabbit (for Immunocytochemistry) ⇒ details 50 μg 019-19741 Keep at -20°C.
Anti Iba1, Rabbit (for Wstern Blotting) ⇒ details 50 μg 016-20001
Anti Iba1, Rabbit, Biotin-conjugated ⇒ details 100 μL 016-26461 Keep at 2-10°C.
Anti Iba1, Rabbit, Red Fluorochrome (635)-conjugated ⇒ details 100 μL 013-26471
Product Name Pkg. Size Wako Cat. No. Storage Condition
Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) 50 μg 015-25191 Keep at -20°C.
Anti Phophorylated α-Synuclein, Monoclonal Antibody (pSyn#64),
Biotin-conjugated ⇒ details		 100 μL 010-26481 Keep at 2-10°C.
Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64),
FITC-conjugated ⇒ details		 100 μL 017-26491