产品编号

JP531-10μg
Recombinant Human TNF-β/TNFSF1
10μg

669

JP531-100μg
Recombinant Human TNF-β/TNFSF1
100μg

6138

JP531-500μg
Recombinant Human TNF-β/TNFSF1
500μg

10830

JP531-1mg
Recombinant Human TNF-β/TNFSF1
1mg

–

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

4049

JP1374

E. coli

171aa

18.7kDa

－

About this protein

Name

Recombinant Human TNF-β/TNFSF1 (Recombinant Human Tumor Necrosis Factor-β/TNFSF1; rHuTNF-β/TNFSF1)；重组人肿瘤坏死因子β/TNFS1

Synonyms

LTA; Lymphotoxin alpha; tnfb; TNF-beta; TNFSF1; TNFSF1B; LT; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); TNFBlymphotoxin-alpha; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5pg/ml, corresponding to a specific activity of >2.0×108IU/mg in the presence of actinomycin D.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 0.02% Tween-20.

Endotoxin

Less than 0.1EU/μg of rHuTNF-β/TNFSF1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

TNF-beta, also known as lymphotoxin-alpha (LTalpha) and TNF-alpha are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical effects. Mature TNF-beta/LTalpha and TNF-alpha share approximately 35% protein sequence homology, and the biologically active secreted forms of both proteins are trimers. Human and mouse TNF-beta/LTalpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity. Soluble TNF-beta/LTalpha is a homotrimer in solution. Secreted TNFbeta/LTalpha also complexes with the membrane associated LTbeta/TNFSF3 to generate two types of heterotrimers, LTalpha1/beta2 and LTalpha2/beta1.

Amino Acid Sequence

LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L

包装清单:

产品编号

产品名称

包装

JP531-10μg

Recombinant Human TNF-β/TNFSF1

10μg

JP531-100μg

Recombinant Human TNF-β/TNFSF1

100μg

JP531-500μg

Recombinant Human TNF-β/TNFSF1

500μg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。