产品编号

JJJP5-10μg
Recombinant Human IL-33
10μg

1671

JJJP5-100μg
Recombinant Human IL-33
100μg

10650

JJJP5-500μg
Recombinant Human IL-33
500μg

18780

JJJP5-1mg
Recombinant Human IL-33
1mg

–

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

90865

O95760

E. coli

159aa

17.9kDa

－

About this protein

Name

Recombinant Human IL-33 (Recombinant Human Interleukin-33; rHuIL-33)；重组人白细胞介素33

Synonyms

C9orf26; DVS27; NF-HEV; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZJP56H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, 150mM NaCl, 1mM EDTA, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-33 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IL-33, encoded by IL-33 gene located on the Chr.9 in humans, is a 30kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20%a.a. sequence identity with other members. IL-33 secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. It elicits its biological effects by interacting with IL1RL1/JJP20 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 mature protein has 52-58%a.a. sequence identity with mouse and rat IL-33.

Amino Acid Sequence

SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET

包装清单:

产品编号

产品名称

包装

JJJP5-10μg

Recombinant Human IL-33

10μg

JJJP5-100μg

Recombinant Human IL-33

100μg

JJJP5-500μg

Recombinant Human IL-33

500μg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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为了您的安全和健康，请穿实验服并戴一次性手套操作。