产品编号

JJJP5-5μg
Recombinant Human IL-8, 77a.a/CXCL8
5μg

669

JJJP5-100μg
Recombinant Human IL-8, 77a.a/CXCL8
100μg

6138

JJJP5-1mg
Recombinant Human IL-8, 77a.a/CXCL8
1mg

19650

Species

Gene ID

Accession

Source

Length

MW

Tag

Human

3576

JP10145

E. coli

77aa

8.9kDa

－

About this protein

Name

Recombinant Human IL-8, 77a.a/CXCL8 (Recombinant Human Interleukin-8, 77a.a./CXCL8; rHuIL-8, 77a.a./CXCL8)；重组人白细胞介素8，77a.a./CXCL8

Synonyms

GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-8, 77a.a./CXCL8 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 (72a.a.) has a 5-10 fold higher activity on neutrophil activation, compared to IL-8 (77a.a.). IL-8 is often associated with inflammation, it has been cited as a proinflammatory mediator in gingivitis and psoriasis.

Amino Acid Sequence

AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS

包装清单:

产品编号

产品名称

包装

JJJP5-5μg

Recombinant Human IL-8, 77a.a/CXCL8

5μg

JJJP5-25μg

Recombinant Human IL-8, 77a.a/CXCL8

25μg

JJJP5-100μg

Recombinant Human IL-8, 77a.a/CXCL8

100μg

JJJP5-1mg

Recombinant Human IL-8, 77a.a/CXCL8

1mg

–

说明书

1份

保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。